Journal of Cardiac Failure
Volume 16, Issue 4 , Pages 357-366, April 2010

Effects of Adipose Tissue-Derived Stem Cell Therapy After Myocardial Infarction: Impact of the Route of Administration

  • Montserrat Rigol, MVD, PhD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • Ciber de Enfermedades Respiratorias (CIBERES), Mallorca, Spain
    • Corresponding Author InformationReprint requests: Montserrat Rigol, MVD, PhD, Institut de Clínic del Tòrax, Hospital Clínic de Barcelona, Villarroel, 170, 08036 Barcelona, Spain. Tel: 34-93-2279305; Fax: 34-93-4514148.
    • ,
  • Núria Solanes, MVD, PhD

      Affiliations

    • Both authors contributed equally to this study.
    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • Jordi Farré, PhD

      Affiliations

    • ICREC Program, Cardiology Service, Hospital de la Santa Creu i Sant Pau, ICCC, Barcelona, Spain
    • ,
  • Santiago Roura, PhD

      Affiliations

    • ICREC Program, Cardiology Service, Hospital de la Santa Creu i Sant Pau, ICCC, Barcelona, Spain
    • ,
  • Mercè Roqué, MD, PhD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • Antonio Berruezo, MD, PhD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • Neus Bellera, MD

      Affiliations

    • Servei de Cardiologia, Hospital de Vall d'Hebron, Barcelona, Spain
    • ,
  • Laura Novensà, MVD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • David Tamborero, BENG, PhD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • Cristina Prat-Vidal, PhD

      Affiliations

    • ICREC Program, Cardiology Service, Hospital de la Santa Creu i Sant Pau, ICCC, Barcelona, Spain
    • ,
  • MA Ángeles Huzman, MVD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • Montserrat Batlle, PhD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • Margo Hoefsloot, MD

      Affiliations

    • Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands
    • ,
  • Marta Sitges, MD, PhD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • José Ramírez, MD, PhD

      Affiliations

    • Centre de Diagnòstic Biomèdic, Hospital Clínic, Barcelona, Spain
    • ,
  • Ana Paula Dantas, PhD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • Anna Merino, MD, PhD

      Affiliations

    • Centre de Diagnòstic Biomèdic, Hospital Clínic, Barcelona, Spain
    • ,
  • Ginés Sanz, MD, PhD

      Affiliations

    • Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain
    • ,
  • Josep Brugada, MD, PhD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain
    • ,
  • Antoni Bayés-Genís, MD, PhD

      Affiliations

    • ICREC Program, Cardiology Service, Hospital de la Santa Creu i Sant Pau, ICCC, Barcelona, Spain
    • ,
  • Magda Heras, MD, PhD

      Affiliations

    • Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Hospital Clínic, Barcelona, Spain

Received 3 September 2009; received in revised form 26 November 2009; accepted 9 December 2009. published online 08 February 2010.

Abstract 

Background

Cell-based therapies offer a promising approach to reducing the short-term mortality rate associated with heart failure after a myocardial infarction. The aim of the study was to analyze histological and functional effects of adipose tissue-derived stem cells (ADSCs) after myocardial infarction and compare 2 types of administration pathways.

Methods and Results

ADSCs from 28 pigs were labeled by transfection. Animals that survived myocardial infarction (n = 19) received: intracoronary culture media (n = 4); intracoronary ADSCs (n = 5); transendocardial culture media (n = 4); or transendocardial ADSCs (n = 6). At 3 weeks' follow-up, intracoronary and transendocardial administration of ADSCs resulted in similar rates of engrafted cells (0.85 [0.19-1.97] versus 2 [1-2] labeled cells/cm2, respectively; P = NS) and some of those cells expressed smooth muscle cell markers. The intracoronary administration of ADSCs was more effective in increasing the number of small vessels than transendocardial administration (223 ± 40 versus 168 ± 35 vessels/mm2; P < .05). Ejection fraction was not modified by stem cell therapy.

Conclusions

This is the first study to compare intracoronary and transendocardial administration of autologous ADSCs in a porcine model of myocardial infarction. Both pathways of ADSCs delivery are feasible, producing a similar number of engrafted and differentiated cells, although intracoronary administration was more effective in increasing neovascularization.

Key Words: Experimental study, intracoronary, transendocardial, neovascularization

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 Supported by grants from Fondo de Investigación Sanitaria [PI060110]; Sociedad Española de Cardiología [2006]; Societat Catalana de Cardiologia [2006]; Hipertensión Esencial: Red de Análisis de Canales iónicos de la musculatura Lisa arterial y su Explotación terapéutica Sistemática [HERACLES, RD06/0009/0008]; Ciber de Enfermedades Respiratorias [06/06/0028], Red temática de investigación cooperativa de la salud [RD06/0003/0008], Fundació Marató TV3 (2007, Malaltires Cardiovascular) and SAF2008-05114-C02-01

PII: S1071-9164(09)01217-2

doi:10.1016/j.cardfail.2009.12.006

Journal of Cardiac Failure
Volume 16, Issue 4 , Pages 357-366, April 2010

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